I don’t know how to handle this Biology question and need guidance.
Upon completion of this lab
exercise
, you should be able to
:
Describe how Coomassie brillian
t
blue G
–
250 dye can be used to detect proteins in
solution
Determine how a set of known protein sample concentrations can be utilized to
determine the concentration of an unknown sample through the use of
a Coomassie
colori
metric assay
Identify the unknown sample dilution(s) that allow
s
you to most confidently determine
the protein concentration
of your unknown
Calculate the unknown sample’s concentration taking into consideration dilution factors
,
where needed
Identify pot
ential sources of variation
in the experimental procedure and establish
techniques to help minimize experimental error
Suggest when to use serial vs parallel dilutions and why each can be beneficial
depending
on the
particular experimental conditions
