I don’t know how to handle this Biology question and need guidance.

Upon completion of this lab


, you should be able to


Describe how Coomassie brillian


blue G

250 dye can be used to detect proteins in


Determine how a set of known protein sample concentrations can be utilized to

determine the concentration of an unknown sample through the use of

a Coomassie


metric assay

Identify the unknown sample dilution(s) that allow


you to most confidently determine

the protein concentration

of your unknown

Calculate the unknown sample’s concentration taking into consideration dilution factors


where needed

Identify pot

ential sources of variation

in the experimental procedure and establish

techniques to help minimize experimental error

Suggest when to use serial vs parallel dilutions and why each can be beneficial


on the

particular experimental conditions



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